We generate murine and rabbit monoclonal antibody following a step-wise approach. We immunize animals from various antigens such as peptides, proteins, bacteria, and inactivated viruses amongst others. Hybridomas are generated by the fusion of splenocytes from the best responding animal with a myeloma cell line. Selected hybridomas are stored in liquid nitrogen after isotyping.
DBS has developed an efficient process for in vitro production of monoclonal antibodies using shake flask, roller bottle, and hollow fiber bioreactor. The process includes evaluation and optimization through clonality, cell line adaptation, mycoplasma testing, production optimization and validation. We have a scalable production capacity from milligrams to grams with stringent quality control, full documentation and international quality standards.